Séminaire IRAMAT-CRP2A /CBMN du 19 décembre 2017 : Art and Cultural Heritage natural polymers by bottom up and top down approaches19/12 : 14h
Par le professeur Caroline Tokarski, MSAP (Miniaturisation pour l’Analyse, la Synthèse & la Protéomique), USR CNRS 3290, Université de Lille 1 Sciences et Technologies, 59655 Villeneuve d’Ascq, France.
Mardi 19 décembre à 14h00
Amphi de la Maison de l’Archéologie
Esplanade des Antilles – Université Bordeaux Montaigne
Art and Cultural Heritage natural polymers by bottom up and top down approaches
The study of organic matter in Cultural Heritage samples is a real analytical challenge. Organic compounds are included in a complex matrix. They are submitted to environmental stress, denaturation and ageing for years. Finally, very low sample amounts are available for analysis. Considering artworks, characterization of organic compounds is an invaluable source of information for knowledge, understanding of a manufacturing process or comprehension of an artist’s technique. This information is also of main importance to propose adapted conservation conditions and best preservation/restoration treatments. In the case of archaeological and paleontological samples, information related to proteins may inform on species phylogeny, evolutionary links but also human habits.
Until recently, the organic compounds from Cultural Heritage samples were analyzed via their constitutive moieties (e.g. amino acids, fatty acids, monosaccharides) inducing a loss of information. In the early 2000s, we have successfully adapted proteomic (bottom up approach) and lipidomic methodologies to the study of few micrograms of historic art paintings or archaeological samples allowing for the first time the accurate structural identification as well as the identification of the biological species. These methodologies are currently used in the most famous international museums (e.g. Metropolitan Museum of Art, New York; Harvard Art Museum in Cambridge).